Fig. 5: Glycosphingolipid biosynthesis pathway is selectively upregulated in Th17 and iTreg cells compared to Th0, Th1 and Th2 cells. | Communications Biology

Fig. 5: Glycosphingolipid biosynthesis pathway is selectively upregulated in Th17 and iTreg cells compared to Th0, Th1 and Th2 cells.

From: The integration of metabolic and proteomic data uncovers an augmentation of the sphingolipid biosynthesis pathway during T-cell differentiation

Fig. 5

a, b PCA depicts profiles of lipid (a) or protein related to lipid metabolism (b) in Th0, Th1, Th2, Th17, and iTregs cells. c, Heatmap depicts the expression levels of each lipid species in Th1, Th2, Th17, and iTreg cells compared to Th0 cells. d, Relative levels of 3-ketosphinanine was shown in Th1, Th2, Th17, and iTreg cells compared to Th0 cells. e, Lipidomics analysis shows the relative contents of lipid species related to ceramide glycosphingolipid in Th1, Th2, Th17, and iTreg cells compared to Th0 cells. f-h, Heatmap depicts the expression levels of each ceramide species (f), each glycol-ceramide species (g), or each ganglioside species (h) was shown in Th1, Th2, Th17, and iTreg cells compared to Th0 cells. i, Cell number of Th17 or iTreg cells treated with DMSO or 2.5 μM GENZ-123346 was shown. j, Naive CD4+ T cells were labelled with e670 proliferation dye and stimulated with immobilized anti-TCRβ mAb and anti-CD28 mAb in the presence of GENZ-123346 under Th17 or iTreg polarization condition. k, BrdU incorporation by Th17 or iTreg cells treated with GENZ-123346 was examined by flow cytometry. l, Intracellular staining of IL-17A and IL-17F in Th17 cells treated with myriocin was shown. m, Surface staining of PD-1 in iTreg cells treated with GENZ-123346 was shown. Four biological replicate was prepared for metabolomics and proteomics analysis. Three independent experiments were performed and showed similar results (i-m). Error bar indicates SD. The source data for the figures is provided in Supplementary Data 1 and Supplementary Data 2.

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