Fig. 4: Comparison of recombinant LBA protein temporal production between FDHY23 and LHP1044 under shake flask cultivation and industrial fermentation conditions.

a Growth curves of strains FDHY23 and LHP1044 during recombinant LBA production under shake flask cultivation at 30 °C. b Expressions of recombinant LBA protein under shake flask cultivation at 30 °C. LBA production was analyzed at 24 h, 36 h, 48 h, 60 h, and 72 h by SDS-PAGE. c Growth curves during high-density fermentation process at 32 °C. d Recombinant LBA protein yield during high-density fermentation with inorganic salts. LBA production was analyzed at 12 h, 24 h, 36 h, 48 h, 60 h, and 72 h by SDS-PAGE. For growth curves, values were calculated as average±SD from three biological replicates. The red and blue colors represent strains FDHY23 and LHP1044, respectively.