Fig. 6: The CYR1N1546K mutation affects adenylate cyclase activity and intracellular cAMP levels. | Communications Biology

Fig. 6: The CYR1N1546K mutation affects adenylate cyclase activity and intracellular cAMP levels.

From: Coupling thermotolerance and high production of recombinant protein by CYR1N1546K mutation via cAMP signaling cascades

Fig. 6

a Conserved domain containing the mutation position. It depicts the arrangement of conserved domains along the amino acid sequence of K.marxianus’ CYR1 gene. The mutation site at position 1546 is situated within the catalytic domain (CYCc) of the CYR1 gene. b The enzymatic activity of adenylate cyclase measured at 30 °C during the mid-log phase and stationary phase of strains FDHY23, LHP1044-CYR1N1546K, and LHP1044. Four biological replicates. c cAMP levels measured at 30 °C during mid-log phase and stationary phase of these three strains. Three biological replicates. d cAMP levels measured at 46 °C during the mid-log and stationary phases of strains FDHY23 and LHP1044. Three biological replicates. e cAMP levels measured at 30 °C during the stationary phase of strains FDHY23 and LHP1044 producing recombinant protein LBA. Four biological replicates. Values were calculated as average±SD from biological replicates. *: p < 0.05; **: p < 0.01; ***: p < 0.001.

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