Fig. 1: Formation of albicin inhibited C3bBb.
From: Mechanism of complement inhibition by a mosquito protein revealed through cryo-EM
Size exclusion chromatography on Superdex 200 of reaction products after incubation of C3b, FB and albicin with FD. a Comparison of complex formation after 1 h (red) and 14 h (blue). b SDS-PAGE of fractions from panel a, red trace. The dimeric peak (C3bBb-albicin) contains all components of the complex. c SDS-PAGE of fractions from panel a, blue trace. Highest molecular weight peak contains only C3b. d Size exclusion traces showing a C3b-albicin mixture (red) and fully dissociated C3bBb-albicin (blue). Albicin does not induce significant dimerization of C3b. e Formation of C3bB in the presence of Ni2+ and albicin. f SDS-PAGE of fractions from panel e. C3b and FB but not albicin are seen in the peak labeled C3bB.
