Table 2 PCR primer sequences
Gene name | Sequence(5’→3’) | |
|---|---|---|
qPCR primers | ||
Rn18s | Fwd | CGGACAGGATTGACAGATTG |
| Â | Rev | CAAATCGCTCCACCAACTAA |
Egr2 | Fwd | CTACCCGGTGGAAGACCTC |
| Â | Rev | AATGTTGATCATGCCATCTCC |
Timp1 | Fwd | GCAAAGAGCTTTCTCAAAGACC |
| Â | Rev | AGGGATAGATAAACAGGGAAACACT |
Col1a1 | Fwd | CATGTTCAGCTTTGTGGACCT |
| Â | Rev | GCAGCTGACTTCAGGGATGT |
Acta2 | Fwd | GACACCACCCACCCAGAGT |
| Â | Rev | ACATAGCTGGAGCAGCGTCT |
Tgfb1 | Fwd | GGACTCTCCACCTGCAAGAC |
| Â | Rev | GACTGGCGAGCCTTAGTTTG |
Tnf | Fwd | TCTTCTCATTCCTGCTTGTGG |
| Â | Rev | GGTCTGGGCCATAGAACTGA |
Il1b | Fwd | GGATGAGGACATGAGCACCT |
| Â | Rev | AGCTCATATGGGTCCGACAG |
Spp1 | Fwd | GGAAACCAGCCAAGGTAAGC |
| Â | Rev | TGCCAATCTCATGGTCGTAG |
Cd9 | Fwd | GATATTCGCCATTGAGATAGCC |
| Â | Rev | TGGTAGGTGTCCTTGTAAAACTCC |
Fabp5 | Fwd | TGAAAGAGCTAGGAGTAGGACTG |
| Â | Rev | CTCTCGGTTTTGACCGTGATG |
Trem2 | Fwd | TGGGACCTCTCCACCAGTT |
| Â | Rev | GTGGTGTTGAGGGCTTGG |
Mmp12 | Fwd | TCAATTGGAATATGACCCCCTG |
| Â | Rev | ACCAGCAAGCACCCTTCACTAC |
F7 | Fwd | AATGAGCAGCTGATCTGTGC |
| Â | Rev | GCAGGACACCTCATCTGGCT |
Atp6v0d2 | Fwd | AAGCCTTTGTTTGACGCTGT |
| Â | Rev | TGAATGCCAGCACATTCATC |
Ctsk | Fwd | GCAGAGGTGTGTACTATGA |
| Â | Rev | GCAGGCGTTGTTCTTATT |
Cd36 | Fwd | GGAGCCATCTTTGAGCCTTCA |
| Â | Rev | GAACCAAACTGAGGAATGGATCT |
Lpl | Fwd | ACAAGGTCAGAGCCAAGAGAAG |
| Â | Rev | TGGTTGTGTTGCTTGCCATC |
Cd207 | Fwd | CCGAAGCGCACTTCACAGT |
| Â | Rev | GCAGATACAGAGAGGTTTCCTTA |
Clec1b | Fwd | GTGATGGCTTTAGTTCTGCTGAT |
| Â | Rev | CTTTTGCTGTGTGACCGACA |
Clec4f | Fwd | GAGGCCGAGCTGAACAGAG |
| Â | Rev | TGTGAAGCCACCACAAAAAGAG |
Cd163 | Fwd | CCTGGATCATCTGTGACAACA |
| Â | Rev | TCCACACGTCCAGAACAGTC |
Nr1h3 | Fwd | GAAATGCCAGGAGTGTCGAC |
| Â | Rev | AAGCGGATCTGTTCTTCTGACAG |
Atf3 | Fwd | CTCTGCCATCGGATGTCCTC |
| Â | Rev | GTTTCGACACTTGGCAGCAG |
Bhlhe40 | Fwd | GACCGGATTAACGAGTGCAT |
| Â | Rev | TGCTTTCACGTGCTTCAACG |
Pparg | Fwd | TGTGGGGATAAAGCATCAGGC |
| Â | Rev | CCGGCAGTTAAGATCACACCTAT |
Rbpj | Fwd | ATGGACTACTCGGAGGGCTT |
| Â | Rev | AGCACTGTTTGATCCCCTCG |
Nfil3 | Fwd | GAACTCTGCCTTAGCTGAGGT |
| Â | Rev | ATTCCCGTTTTCTCCGACACG |
Genotyping primers | ||
Lyzs_pro | Â | GCATTGCAGACTAGCTAAAGGCAG |
Lyzs_ex1_r | Â | GTCGGCCAGGCTGACTCCATAG |
Cre8 | Â | CCCAGAAATGCCAGATTACG |
Egr2_flox_p4 | Â | GGGAGCGAAGCTACTCGGATACGG |
Egr2_flox_p5 | Â | GTTGCTCTGTAGTGTTGGAATCATG |
