Fig. 4: OliTag-seq Sensitivity in Off-Target Detection vs. GUIDE-seq.
From: OliTag-seq enhances in cellulo detection of CRISPR-Cas9 off-targets
a–d Comparative Site ldentification: These panels compare off-target sites detected by OliTag-seq and GUIDE-seq for sgRNAs targeting EMX1 (a), VEGF1 (b), VEGF2 (c), and VEGF3 (d) in U2OS cells. Next to each listed site, the respective read counts from OliTag-seq are indicated, showcasing the technique’s sensitivity. Only a selection of off-targets are shown for VEGF1, VEGF2, and VEGF3, with a comprehensive list available in Supplementary Data 1. The data for GUIDE-seq were obtained from a previous publication. e Overlap Analysis of Off-Target Sites: Venn diagrams demonstrate the shared and unique off-target sites identified by GUIDE-seq and OliTag-seq for each sgRNA. This visual comparison underscores the enhanced detection capability of OliTag-seq.
