Fig. 6: Off-Target Detection Boosted by Stable Cas9 in iPSCs.
From: OliTag-seq enhances in cellulo detection of CRISPR-Cas9 off-targets
a EMX1 Off-Target Site Mapping in iPSCs: Depicts off-target sites for EMX1 in iPSCs, contrasting outcomes from cells with transient versus stable Cas9 expression. b VEGF3 Off-Target Visualization in iPSCs: Shows VEGF3 off-target sites in iPSCs, again comparing transient and constitutive Cas9 expression. The sequence details for VEGF3 are abridged, with a comprehensive account in Supplementary Data 3. Off-index values, representing the ratio of off-target to on-target reads, are presented below each visualization. c Comparative Off-Target Site Analysis for EMX1 and VEGF3: A Venn diagram summarizes the off-target sites for EMX1 (left) and VEGF3 (right) identified in iPSCs with transient or stable Cas9 expression, illustrating the differential detection profiles.iPSC with constitutive Cas9 expression enhance off-target identification.
