Fig. 8: Off-Target Mapping in CAR-T Loci via OliTag-seq.
From: OliTag-seq enhances in cellulo detection of CRISPR-Cas9 off-targets
a–e Depicts off-target detection in CAR-T cell therapy-related genes using OliTag-seq. Shown are sgRNAs aimed at TRAC-CJ (a), TRAC-MH (b), TRBC-CJ (c), TRBC-MH (d), and PDCD1-CJ (e) in the iPSC-Cas9 cell line using a complex of crRNA:tracrRNA. TSA (0.1 pM) was applied overnight after electroporation to enhance detection. Mismatched sequences at off-target sites are color-coded. Read counts from OliTag-seq are annotated beside each target. On-target sites are denoted with black squares, and off-targets previously identified by iGUIDE are marked with open diamonds.
