Fig. 4: Simultaneous evaluation of epitope similarity using two rAbs. | Communications Biology

Fig. 4: Simultaneous evaluation of epitope similarity using two rAbs.

From: Epitope binning for multiple antibodies simultaneously using mammalian cell display and DNA sequencing

Fig. 4

a Diagram of the cDNA constructs in the plasmids pCSII/HER2-mCherry and pCSII/scFv-BFP for expressing HER2 and displaying scFv on the cell surface, respectively. ss, secretion signal peptide; H6, His6 tag peptide; L, linker peptide; TM, transmembrane domain. b mCherry and BFP expression in K562/HER2/scFv(Dac)B, K562/HER2/scFv(Per)B, and K562/HER2/scFv(Tra)B cells. Scale bar = 50 μm. c Epitope similarity evaluation between qAb and two rAbs. K562/HER2/scFv(Dac)B, K562/HER2/scFv(Per)B, and K562/HER2/scFv(Tra)B cells were incubated with both 1 nM Pert-AF647 and 1 nM Tras-AF488 prior to FCM analysis. Representative contour plots are shown. d Epitope similarity evaluation between two qAbs and two rAbs. K562/HER2/scFv(Dac)B, K562/HER2/scFv(Per)B, and K562/HER2/scFv(Tra)B cells were mixed pairwise in a 1:1 ratio and incubated with both 1 nM Pert-AF647 and 1 nM Tras-AF488 prior to FCM analysis. Representative contour plots are shown.

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