Fig. 5: Evaluation of qAbs with various HER2-binding affinities.
a HER2-binding affinity of qAbs. KD ± SEM values are shown. n = 3. b Epitope similarity evaluation between qAbs and Pert-AF647 at 0.1 nM. K562/HER2 cells displaying Per, Per mutants, and Dac were incubated with 0.1 nM Pert-AF647 prior to FCM analysis. Representative contour plots are shown. Percentage of Pert-AF647-non-binding cells calculated using FCM plots from three independent experiments is also shown as the mean ± SEM. n = 3. c Epitope similarity evaluation between qAb and Pert-AF647 at 10 nM. K562/HER2 cells displaying Per, Per mutants, and Dac were incubated with 10 nM Pert-AF647 prior to FCM analysis. Representative contour plots are shown. Percentage of Pert-AF647-non-binding cells calculated using FCM plots from three independent experiments is also shown as the mean ± SEM. n = 3.
