Fig. 2: Phenotypic characterization of A. fumigatus sirtuin mutants.
From: Sirtuin E deacetylase is required for full virulence of Aspergillus fumigatus
A A. fumigatus mutants inoculated on YAG medium for 120 h at 37 °C. B Relative quantification of mutant strains growth based on colony diameter in the presence of calcofluor white (30 µg/mL, n = 6), C congo red (50 µg/mL, n = 6), and D caspofungin (1 µg/mL, n = 3). E Relative quantification based on feature area of cell wall monosaccharides (glucose, mannose, glucosamine, and galactose) from WT and sirtuin mutant mycelia after acid (H2SO4) treatment (n = 3). 1 mg/mL of fucose was used as an internal control for normalization. F A. fumigatus sirtuin mutants grown on glucose minimal media (GMM) for 120 h at different temperatures. Significant differences were observed by using two-way ANOVA. *p < 0.05, **p < 0.002, ***p < 0.001 and ****p < 0.0001 indicate significant differences from comparisons to the WT strain.
