Fig. 5: Decondensation of chromatin structure facilitates the release of nuclear dsDNA into the cytoplasm. | Communications Biology

Fig. 5: Decondensation of chromatin structure facilitates the release of nuclear dsDNA into the cytoplasm.

From: Super-resolution imaging of T lymphocyte activation reveals chromatin decondensation and disrupted nuclear envelope

Fig. 5

a Representative conventional wide-field fluorescence image and STORM image of genomic DNA in activated T cells. b Representative STORM images of genomic DNA after 24 and 48 h of stimulation, respectively, as shown along the horizontal row. Green arrows indicate the regions where the cytosolic DNA is mainly located close to the disrupted chromatin region at the periphery, compared to the more compacted regions of chromatin (yellow arrows). Scale bars: 2 µm and 1 µm in the original and magnified images, respectively. c DNA nanodomain size in the nucleus with different stimulation time (n = 151, 65 and 220 cells, respectively). d Correlation between the size of nuclear DNA nanodomains and cytosolic DNA nanocluster size (Pearson’s r = 0.3). e Correlation between the size of nuclear DNA nanodomains and the number of cytosolic DNA nanoclusters (Pearson’s r = −0.46).

Back to article page