Fig. 2: Increased detection sensitivity of spontaneous [Ca2+]in transients by ultrahigh-affinity GECIs. | Communications Biology

Fig. 2: Increased detection sensitivity of spontaneous [Ca2+]in transients by ultrahigh-affinity GECIs.

From: High-affinity tuning of single fluorescent protein-type indicators by flexible linker length optimization in topology mutant

Fig. 2

a The measurement of [Ca2+]in dynamics induced by bath application of 10 μM [cAMP]ex at t = 30 s (left) or spontaneously generated [cAMP]ex in the population of chemotactic cells (right). The intensity ratio of GECIs over the fused reference maker was shown for quantitative analysis. Mean ±s.e.m. of 10 cells for left. Population average in ROIs containing ~10 cells was shown for three ROIs for right. b Signaling waves of [Ca2+]in in a cell population cultured on a non-nutrient agar plate. Raw GECI’s intensity (left), intensity ratio (GECI/reference, middle). Scale bar: 0.1 mm. c, d The magnitude of signal change, defined as the ratio of a given response to the maximum signal change in each GECI (c), and the peak signal-to-noise-ratio. c And the peak signal-to-noise-ratio (SNR, d) for spontaneous [Ca2+]in. See Supplementary Fig. 8a for the quantification method used in Fig. 2c. Mean ± s.e.m. of 5 measurements. e Tau of the signal decay in (a). Mean ± s.e.m of 10 cells.

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