Fig. 4: Triple-function imaging of Ca2+, cGMP, and cAMP. | Communications Biology

Fig. 4: Triple-function imaging of Ca2+, cGMP, and cAMP.

From: High-affinity tuning of single fluorescent protein-type indicators by flexible linker length optimization in topology mutant

Fig. 4

a Schematic illustration of triple-function imaging in cells simultaneously expressing indicators for Ca2+, cGMP, and cAMP. b Fluorescence signal changes of BGECO-nano, Green cGull, and R-FlincA during three cycles of wave propagation. The 3-frame-moving averaged data in ROI containing ~10 cells on a non-nutrient agar plate was shown. Open and closed arrowheads indicate the timing of rise and peak. c A close-up view of the signaling wave at the traveling front (box in b). Scale bar: 0.1 mm.

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