Table 1 Literature evidence supporting the roles of TFs and TF pairs, as predicted by GEMSTAT models
From: Mechanistic analysis of enhancer sequences in the estrogen receptor transcriptional program
TF | GEMSTAT inferred role | % enhancer affected | Avg. % change | Literature-suggested role for TF | Reference PMID |
|---|---|---|---|---|---|
ERα | Strong activator | 63.8 | −32.5 | Transcriptional activator | 26884552 |
FOXA1 | Weak activator | 47.1 | −20.0 | Pioneer factor. Alters DNA accessibility profile and is necessary for Estrogen-induced ERα binding. | 18358809,16009131, 21151129 |
NKX3-1 | Weak inhibitor | 22.4 | 16.0 | Represses ERα activity by competitively binding to chromatin/recruiting HDAC1 | 18794125 |
NR5A2 | Strong activator | 26.7 | −20.2 | Activates ERα-mediated transcription at least partly through co-binding to Estrogen Response Elements. | 22359603 |
PBX1 | Weak activator | 6.4 | −24.7 | Pioneer factor | 22125492 |
PGR | Strong/Weak inhibitor | 6.4 | −9.2 | Unclear/controversial | 26153859,28729413, 27885264 |
RELA (NFκb) | Ambiguous (weak activator/strong inhibitor) | 9.3 | −13.1 | Transcriptional activation; controversial since ERα is known to repress NFκb transcriptional activity | 25752574,20705611, 19920189,18703630, 22963717 |
RUNX1 | Weak activator/Strong binding | 10.55 | −6.75 | Mediates indirect binding (tethered) of ER to DNA | 20547749 |
SP1 | Weak activator | 18.8 | −8.7 | Transcriptional activation in part due to mediation of indirect ERα-DNA binding | 9328340,11250935, 11345900 |
AP2-γ | Strong Activator | 9.0 | −12.8 | Pioneer factor | 21572391 |
YBX1 | Weak/Strong inhibitor | 9.8 | 10.1 | Transcriptional repression through direct interaction with ERα | 29180470 |
ERα:FOXA1 | Cooperative | 17.8 | −17.7 | Cooperative activity essential for ERα activity | 18358809,16009131, 21151129 |
