Fig. 6: CASP4 is induced by IFN-γ.
From: Caspase-4 promotes metastasis and interferon-γ-induced pyroptosis in lung adenocarcinoma
a Identification of the top 133 genes (Spearman’s correlation >0.4) coexpressed with CASP4 using the TGCA-LUAD dataset. b The top 133 genes coexpressed with CASP4 were analyzed via TRRUST analysis to determine the transcription factors involved. The horizontal axis shows accumulative hypergeometric p-values. c CASP4 mRNA induction by IFN-γ stimulation in NCI-H292 cells. Cells were treated for 24 h. d Immunoblotting analysis of CASP4 in NCI-H292 cells treated with PBS and 1 ng/mL of IFN-γ. Cells were treated for 48 h. e Evaluation of CASP4 mRNA expression in NCI-H292 cells by RT-qPCR and assessment of cell viability by CCK-8 assay after treatment with IFN-γ at different concentrations. The samples for RT-qPCR and CCK-8 assay were collected after 24 h and 72 h, respectively. f Comparison of CASP4 mRNA expression by RT-qPCR between mock and CASP4 OE after treatment with high-dose IFN-γ (100 ng/mL) for 24 h. g Downregulation of CASP4 mRNA expression by STAT1 knockdown (shSTAT1) in NCI-H292 cells. h Representative images of immunoblotting analysis of CASP4 and STAT1 protein in mock and shSTAT1 NCI-H292 cells. n.s.: not significant; N.S: nonspecific. Data are expressed as mean ± standard deviation (n = 3). *P < 0.05, **P < 0.01 compared with PBS treatment. Densitometry values are shown above each blot.
