Fig. 2: In vivo results at 400 ms exposure time, N = 12 biologically independent subjects.

a Mean and 95% confidence intervals (CI) of the plot profiles that measure the fluorescence intensity of the fluorophore infusion from the surface of the brain, identified in the plots as distance = 0.00 mm, perpendicularly towards inside the brain. b Distribution of fluorophore infusion distance, measured from the surface of the brain to the point where the fluorescence intensity reached 10% of the maximum intensity (d₁₀) and 30% of the maximum intensity (d₃₀). c Displacement from the surface of the brain, for the highest value of fluorescence intensity in each trial. d Area covered by fluorophore infusion, at a threshold of 10% of the maximum intensity (A₁₀) and 30% of the maximum intensity (A₃₀). e Fluorescence peak intensity value in each trial, as a percentage of the highest peak in all trials. The bar graph indicates the mean, and the error bars correspond to the 95% CI. e Fluorescence peak intensity value in each trial, as a percentage of the highest peak in all trials. f Fluorescence images overlaid upon brightfield images for the brain slice selected for analysis, with the intensity threshold set at 10%, and g the intensity threshold set at 30% of maximum intensity. Significant differences are indicated by ** for p < 0.01, and * for p < 0.05.