Fig. 1: Single-cell RNA-seq profiling of epithelial cell in DSS-induced mouse colitis. | Communications Biology

Fig. 1: Single-cell RNA-seq profiling of epithelial cell in DSS-induced mouse colitis.

From: Integrative analysis of single-cell RNA-seq and gut microbiome metabarcoding data elucidates macrophage dysfunction in mice with DSS-induced ulcerative colitis

Fig. 1

a Mice were treated with 3% DSS for 6 days and evaluated at day 11 for acute colitis. The relative body weight of mice treated with 3% DSS and DW. Colitis scores were determined based on clinical parameters such as weight loss, stool consistency, and bleeding. b Histological appearance of the colon at day 11 in mice with 3% DSS compared with DW. c Scheme of the chronic colitis (CC) model induced by 3% DSS. Relative body weight changes during the experiment were measured. Colitis scores were determined based on clinical parameters such as weight loss, stool consistency, and bleeding. d Histological appearance of the colon at the endpoint of the experiment in mice with 3% DSS compared with DW. e UMAP plots displaying cell populations from all participant mice colored by major cell type (upper plot) and by condition (bottom panel; HC indicates healthy conditions, AC acute colitis, and CC chronic colitis. f Bar plots showing proportion of major cell type in each condition. g Dot plot depicting the expression profiles of cell barrier-related genes in epithelial cell population. h Western blotting results showing the protein level expression of intestinal barrier status markers. DW distilled water, DSS dextran sodium sulfate. The uncropped/unedited blot images were added in the Supplementary Information (Supplementary Fig. S5).

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