Fig. 2: Analysis of soluble oligomers of TDP-43 CTFs using fluorescence correlation spectroscopy (FCS). | Communications Biology

Fig. 2: Analysis of soluble oligomers of TDP-43 CTFs using fluorescence correlation spectroscopy (FCS).

From: Hetero-oligomerization of TDP-43 carboxy-terminal fragments with cellular proteins contributes to proteotoxicity

Fig. 2

a Typical photon count rate records during the FCS measurement of GFP monomers (Mo), full-length TDP-43 (FL), and CTFs in Neuro2a cell lysate. The Gray bar on the y-axis shows the range of 400 kHz of photon count rate. b The plot of normalized counts per particle (CPP), and relative fluorescent brightness per single particle. Bars: mean + 95% CI; Dots: raw values (n = 4 independent experiments). c The weighted average of diffusion time was obtained by curve fitting analysis of the autocorrelation function using a two-component diffusion model. Bars: mean and 95% CI; Dots: raw values (n = 4 independent experiments). In b and c, p-values above the bars and lines: one-way ANOVA with Tukey’s test compared to GFP monomers as control and comparison between the lines, respectively.

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