Fig. 4: Co-condensation of C220 and F220 with the glycine-rich region. | Communications Biology

Fig. 4: Co-condensation of C220 and F220 with the glycine-rich region.

From: Hetero-oligomerization of TDP-43 carboxy-terminal fragments with cellular proteins contributes to proteotoxicity

Fig. 4

a Confocal fluorescence and bright field microscopic images of Neuro2a cells expressing GFP-tagged C220 or F220 with the glycine-rich region (GRR; C274) tagged with a red fluorescent protein (RFP). Mo indicates GFP or RFP monomers. White arrows represent C274-positive condensates in the cytoplasm. Bars = 5ā€‰Ī¼m. b The population of Neuro2a cells that contain GFP-positive condensates (conds) in the cytoplasm. c The population of Neuro2a cells containing RFP-positive condensates in the cytoplasm. d The ratio of RFP-positive condensates to GFP-positive ones. In b, c, and d, ND: Not determined; N/A: Not applicable; p-values above the lines: one-way ANOVA with Tukeyā€™s test (nā€‰=ā€‰3 independent experiments).

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