Fig. 5: Co-condensation of C220 and C233 with full-length TDP-43.

a Confocal fluorescence and bright field microscopic images of Neuro2a cells expressing GFP-tagged C220 or C233 with TDP-43 (FL) tagged with a red fluorescent protein (RFP) (TDP43-R). Mo indicates GFP or RFP monomers. White arrowheads represent TDP-43-positive condensates in the cytoplasm. White arrows represent TDP-43-negative condensates in the cytoplasm. Bars = 5 μm. b The population of Neuro2a cells that contain GFP-positive condensates (conds) in the cytoplasm. c The population of Neuro2a cells containing RFP-positive condensates in the cytoplasm. d The ratio of RFP-positive condensates to GFP-positive ones. ND: Not determined; N/A: Not applicable; p-values above the lines: one-way ANOVA with Tukey’s test (n = 3 independent experiments). e Western blot of 1% SDS-soluble (S) and -insoluble (P) cell lysates using an anti-GFP, anti-RFP, and anti-α-tubulin antibody (left). The right arrows represent the migration position of the proteins. GFP monomers: Mo. The quantification of the abundance of TDP43-R in the insoluble (Insol.) fraction. The abundance shows the normalized band intensity in the P fraction to the total (S + P) fraction (right). Bars: mean and 95% CI; Dots: raw values; ND: Not determined; p-values above the lines: one-way ANOVA with Tukey’s test (n = 3 independent experiments).