Fig. 7: The role of the hydrophobic region in the glycine-rich region of TDP-43 to the co-condensation process in Neuro2a cells.

a Domain structure and abbreviation of TDP-43 CTFs: amino acids 220–414 (C220), 274–414 (C274), C220 lacking 311–340 region (C220Δ), and C274 lacking 311-340 region (C274Δ). GRR denotes a glycine-rich region. b Confocal fluorescence and bright field microscopic images of Neuro2a cells co-expressing GFP- and RFP-tagged TDP-43 CTFs. Mo denotes GFP or RFP monomers. White arrows represent cytoplasmic condensates including both GFP- and RFP-tagged CTFs. White arrowheads represent cytoplasmic condensates including only GFP-tagged CTFs. Bars = 5 μm. c, d The population of Neuro2a cells containing GFP-positive and RFP-positive cytoplasmic condensates (conds). e Ratio of cells containing RFP-positive condensates in a group of cells containing GFP-positive condensates. f Western blot of 1% SDS-soluble and -insoluble cell lysates using anti-GFP or anti-RFP antibodies. g The quantification of the abundance of GFP-tagged proteins in the insoluble fraction (Insol.). h The quantification of the abundance of RFP-tagged proteins in the insoluble fraction (Insol.). c, d, e, g, and h Bars: mean and 95% CI; Dots: raw values (n = 3 independent experiments); ND: Not determined; N/A: Not applicable; p-values above the lines: one-way ANOVA with Tukey’s test.