Fig. 8: Intermolecular assembly of C220 and C263 using a blue light-induced oligomerization tag derived from a cryptochrome variant in Neuro2a cells. | Communications Biology

Fig. 8: Intermolecular assembly of C220 and C263 using a blue light-induced oligomerization tag derived from a cryptochrome variant in Neuro2a cells.

From: Hetero-oligomerization of TDP-43 carboxy-terminal fragments with cellular proteins contributes to proteotoxicity

Fig. 8

a Fluorescence images of Neuro2a cells expressing TDP-43 CTFs: C220, C220Ī”, C263, and C263Ī” tagged with a cryptochrome-derived oligomerization inducer, CRY2olig (CRY), and RFP before and after the 488 nm-light irradiation. The order of the hyphens before and after indicates the N/C-terminal side of the CRY tag. White arrowheads represent cells with foci formation after the irradiation. Bars = 5 μm. b Population of cells that form foci after the blue-light irradiation obtained from all measured cells (the numbers of independently counted cells were 26 for Lane 1, 27 for Lane 6, 36 for Lanes 2, 5, and 7, 35 for Lanes 3, 4, 10, and 34 for Lanes 8 and 9). ND: Not determined; p-values above the bars and lines: hypothesis test for the difference in the population proportions compared to a tag itself as control and comparison between the lines, respectively; ***p < 0.001. c Schematic diagram of CRY tagged at the N-terminus of TDP-43 CTFs approaching and assembling after light irradiation. d Schematic diagram of CRY tagged at the C-terminus of TDP-43 CTFs approaching but not assembling even after light irradiation.

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