Fig. 1: Correlation between tissue specificity and circadian rhythmicity across mouse organs.
From: Circadian-driven tissue specificity is constrained under caloric restricted feeding conditions
a The heatmaps display the tissue-specificity (measured by SPM) of circadian genes, and the pie charts show the proportion of them in tissue-specific genes (top 10% SPM score). R function pheatmap with the parameter scale = row was used to display the SPM changes of circadian genes. b The number of tissue-specific genes was positively correlated with the number of circadian genes in the 12 examined organs, as seen with tissue-specificity indicators including Tau, EE, and SPM. c Enrichment analysis of circadian Tau genes and rhythmically expressed genes. JTK_CYCLE was used to analyze Tau values of genes at different time points, and genes with Benjamini-Hochberg adjusted P < 0.05 were considered circadian Tau genes. Tissue circadian genes from the mouse circadian transcriptome atlas were also analyzed by JTK_CYCLE. d Enrichment analysis of tissue-specific genes (SPM) and tissue-specific genes (Tau). Tissue-specific genes were identified using the SPM value and Tau value calculated from the average gene expression in the mouse circadian transcriptome atlas and a cutoff value > 0.8. Tissue specific genes (Tau) is classified according to the tissue with the highest gene expression. e Enrichment analysis of biological processes of tissue-specific genes across circadian time in the liver. Both odds ratio and significance level (p) are indicated. f The heatmaps display the expression of enriched biological pathways across circadian time in the liver. g Scatter plots showing the relationship between the amplitudes of circadian genes and tissue specificity under normal conditions. In all four organs, tissue-specificity indicators (such as SPM) from the mouse circadian transcriptome atlas are significantly positively correlated with the amplitude of gene expression of the transcriptome (AMP), even in sleep deprivation conditions. Each dot represents a gene, and the Pearson correlation coefficient and its significance level (P) are displayed in each plot. h Scatter plots showing the relationship between the amplitudes of circadian genes and tissue specificity under sleep deprivation conditions.
