Fig. 2: Overall structure of the BlCas9–sgRNA–target DNA complex.
From: Structure and engineering of Brevibacillus laterosporus Cas9
a Domain structure of BlCas9. The HNH nuclease domain was truncated for crystallization. BH bridge helix, PLL phosphate lock loop. b Diagram of the sgRNA and target DNA used for crystallization. TS target strand, NTS non-target strand. c Overall structure of BlCas9-ΔHNH in complex with the sgRNA and its target DNA. Disordered regions are indicated by dotted lines. d, e Surface representations of the BlCas9–sgRNA–target DNA complex, colored according to the protein domain (d) and electrostatic surface potential (e).
