Fig. 3: Guide RNA architecture and recognition.
From: Structure and engineering of Brevibacillus laterosporus Cas9
a Schematic of the sgRNA and target DNA. Disordered regions are enclosed in gray boxes. b Structure of the sgRNA scaffold. The disordered regions are indicated by dotted lines. c Recognition of the sgRNA scaffold by BlCas9. d–g Recognition of the repeat:antirepeat duplex (d), stem loop 1 (e), stems 1 and 2 (f), and stem loop 2 (g) of the sgRNA scaffold. Hydrogen bonds are depicted with green dashed lines.
