Table 1 Binding of Claudins to cCpE and COP-1 to Claudin/cCpE Complexes

From: Structural and biophysical insights into targeting of claudin-4 by a synthetic antibody fragment

Subtype

cCpE Binding

Subtype/cCpE

COP-1 Binding

KD (nM)

t1/2 (min)

KD1 (nM)

KD2 (µM)

t1/2 (min)

hsCLDN-3

374.7 ± 2.1

5.0

hsCLDN-3/cCpE

339.9 ± 9.4

0.7 ± 1.5

0.1

hsCLDN-4

7.7 ± 0.1

55.0

hsCLDN-4/cCpE

23.4 ± 1.7

8.2 ± 6.6

21.2

hsCLDN-9

4.9 ± 0.1

88.9

hsCLDN-9/cCpE

120.6 ± 0.8

1.4 ± 0.1

2.4

mmCLDN-3

9.0 ± 0.1

25.7

mmCLDN-3/cCpE

47.3 ± 1.0

42.0 ± 24.9

10.6

mmCLDN-4

11.9 ± 0.1

14.1

mmCLDN-4/cCpE

60.5 ± 2.1

22.2 ± 4.0

11.9

  1. BLI was used to measure binding kinetics and affinities. Binding of claudins to cCpE represent a single experiment and was fit with a 1:1 binding model, while COP-1 binding measurements represent averages from duplicate experiments and were fit with a 2:1 heterogenous ligand model, hence two KDs are reported. The t1/2 (ln 2/koff) was calculated from the dissociation rates between claudin/cCpE and COP-1 that represent KD1. The full binding results, including kinetic rates, appear in Supplementary Table 2.
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