Fig. 3: Enhanced CF translocation and impaired elimination of somatic CF synapses in lobules 8-9 but not in lobules 1-4/5 of adult TARPγ2-GC-KO mice. | Communications Biology

Fig. 3: Enhanced CF translocation and impaired elimination of somatic CF synapses in lobules 8-9 but not in lobules 1-4/5 of adult TARPγ2-GC-KO mice.

From: Direct and indirect pathways for heterosynaptic interaction underlying developmental synapse elimination in the mouse cerebellum

Fig. 3

a–d Double immunostaining for VGluT2 (red) and calbindin (green) (a1, b1, c1, d1) and single immunostaining of VGluT2 (a2, b2, c2, d2) in lobule 9 (a, b) and lobule 4/5 (c, d) from a control (a, c) and a TARPγ2-GC-KO (b, d) mouse at 8 weeks of age. Dotted lines indicate the pial surface. Boxed regions in (a1, b1, c1, d1) are enlarged as insets in individual figure panels. Scale bars in (c1) and (d1), 20 μm for the lower magnification images and 5 μm for the higher magnification images. e, f Summary violin plots for the relative height of the tip of VGluT2-positive CF terminals (e), representing the shortest distance from the middle of the PC layer to the distal tip of the VGluT2-positive puncta divided by the molecular layer thickness. n = 282 and 333 in lobules 8-9 and1-4/5, respectively, from 3 control mice; n = 264 and 348 in lobules 8-9 and1-4/5, respectively, from 3 TARPγ2-GC-KO mice) and the number of VGluT2-positive CF terminals around the PC soma (f, representing the number of VGluT2-positive CF terminals per 10 μm length of the PC somatic membrane. n = 120 and 132 in lobules 8-9 and 1-4/5, respectively, from 3 control mice; n = 126 and 137 in lobules 8-9 and1-4/5, respectively, from 3 TARPγ2-GC-KO mice) in lobules 8-9 and lobules 1-4/5 from control (open plots) and TARPγ2-GC-KO (filled plots) mice at 8 weeks of age. The dashed line within each violin plot represents the median, while the solid lines at the top and bottom indicate the 75th and 25th percentiles, respectively. *P < 0.05, ***P < 0.001, t test.

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