Fig. 4: The absence of PRMT2 exacerbates the inflammatory phenotype of mouse bone marrow-derived macrophages.
From: Protein arginine methyltransferase 2 controls inflammatory signaling in acute myeloid leukemia
a Schematic representation for the obtention of BMDMs from purified monocytes from Prmt2–/– or control (Prmt2+/+) mouse BM. b Morphological analysis of monocytes after 0 and 5 days of differentiation into macrophages. Images from phase-contrast microscopy using an Incucyte® S3 Live-Cell Analysis System at magnification x20. Il6 (c) and Tnf (d) expressions measured by RT-QPCR on total RNA from mouse BMDMs after stimulation by 100 ng/mL LPS for 8 h. ELISA on BMDM culture media for IL6 (e) and TNF (f) after stimulation by 100 ng/mL LPS for 8 h. All error bars represent the mean ± SD of three independent experiments. One sample t test with p values indicated in the graphs: *p < 0.05.
