Fig. 5: PRMT2 is involved in the human inflammatory response through the control of STAT3 activation in vitro upon stress.
From: Protein arginine methyltransferase 2 controls inflammatory signaling in acute myeloid leukemia
a RT-QPCR on total RNA measuring IL6 in PRMT2KO and WT cells after stimulation by 100 ng/mL LPS during 4, 8, or 24 h. b ELISA on human IL6 in PRMT2KO and WT cell culture medium after stimulation by LPS (100 ng/mL) during 24 h. Western Blot analysis (c) and quantitation (d) of phosphorylated (Y705) and total forms of STAT3 after stimulation by LPS (100 ng/mL) during 4, 8, 24 or 48 h. GAPDH is used as a loading control. Western Blot analysis (e) and quantitation (f) of phosphorylated and total forms of STAT3 after stimulation by LPS (100 ng/mL) during 8 h of HL-60 WT, KO, or KO cells infected with viruses containing DNA coding for either an empty vector (EV), exogenous PRMT2 (PRMT2) or a catalytically dead mutant (E220Q). GAPDH is used as a loading control. All the presented blots are representative figures from at least three independent experiments. All error bars represent the mean ± SD of three independent experiments. Ctrl: Control. One-way ANOVA (a, d, f) or one sample t test (b) with p values indicated in the graphs: *p < 0.05; **p < 0.01.
