Fig. 6: The absence of PRMT2 deregulates the NF-κB signaling pathway in AML upon stress.
From: Protein arginine methyltransferase 2 controls inflammatory signaling in acute myeloid leukemia
Western Blot analysis (a) and quantitation (b) of phosphorylated (Y705) and total forms of STAT3 after stimulation by IL6 (40 ng/mL) during 0.5, 2, 8 or 24 h, shown as mean ± SD of three independent experiments and analyzed by one-way ANOVA. c Western Blot analysis of phosphorylated and total forms of the SAPK/JNK, ERK1/2, and p38 MAPKs on PRMT2KO and WT cells after stimulation by 100 ng/mL LPS during 4, 8, 24 or 48 h. d Western Blot analysis of NF-κB p65 and phosphorylated (Y705) STAT3 nuclear translocation after cellular fractionation of PRMT2KO and WT HL-60 cells. SP1 and ACTB correspond to nuclear and cytoplasmic controls, respectively. Ctrl: Control. All presented blots are representative figures from three independent experiments.
