Fig. 2: Verification that LcsG is involved in the methylation of leucinostatins. | Communications Biology

Fig. 2: Verification that LcsG is involved in the methylation of leucinostatins.

From: Characterization of a methyltransferase for iterative N-methylation at the leucinostatin termini in Purpureocillium lilacinum

Fig. 2

a SDS‒PAGE analysis of the recombinant protein LcsG. b In vitro LcsG activity analysis using LeuK0 (4) and LeuA (3) as the substrates and the [M + H]+ ions of three new product peaks at positions 6–8. c Time dependency of the variations in each component. d Kinetic analysis of LcsG using LeuA (3, top) and LeuK0 (4, bottom) as substrates. All the data are represented as the means of n = 3 biologically independent samples, and the error bars show the standard deviations (c, d).

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