Fig. 5: Growth behavior of C. crescentus strains upon heterologous expression of E. coli pitA. | Communications Biology

Fig. 5: Growth behavior of C. crescentus strains upon heterologous expression of E. coli pitA.

From: The cytoplasmic phosphate level has a central regulatory role in the phosphate starvation response of Caulobacter crescentus

Fig. 5

Shown are the growth curves of wild-type, ΔphoB and ΔpstS cells lacking pitA (CB15N, JK2, JK158) (- PitA) or carrying pitA under the control of a xylose-inducible promoter (MAB257, MAB258, MAB259) (+PitA). The cultures were grown in PYE medium (+Pi) or pre-grown in PYE medium and then diluted (1:20) into M2G−P medium prior to further incubation (−Pi). For strains carrying pitA, media were supplemented with xylose. Lines represent the average of two independent experiments. The edges of the shades indicate the values obtained in the two measurements.

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