Fig. 6: Effect of PitA production on the activity of different phosphate-responsive promoters. | Communications Biology

Fig. 6: Effect of PitA production on the activity of different phosphate-responsive promoters.

From: The cytoplasmic phosphate level has a central regulatory role in the phosphate starvation response of Caulobacter crescentus

Fig. 6

Wild-type (CB15N), WTpitA (MAB213), ΔpstS (JK158) and ΔpstSpitA (MAB215) cells were transformed with reporter plasmids that carried fusions of (A) the PhoB-dependent pstS (pMAB34) or pstC (pMAB104) promoters or (B) the PhoB-independent ppk1 (pMAB106) or CCNA_01606 (pJR16) promoters to the lacZ gene. Transformants were grown in PYE medium supplemented with xylose and subjected to β-galactosidase activity assays. Data represent the mean (± SD) of three independent measurements.

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