Fig. 8: Genes specifically controlled by PhoB.

A, B Venn diagrams giving the number of differentially regulated genes that are found in common in pairwise comparisons of cells with highly activated PhoB (ΔpstS and ΔpstS^pitA) versus cells with inactive (ΔphoB, ΔphoB^pitA) or poorly activated (WT, WT^pitA) PhoB. Black frames indicate the genes that are significantly regulated in all comparisons shown. The strains analyzed are: ΔpstS (JK158), ΔpstS^pitA (MAB259), ΔphoB (JK2), ΔphoB^pitA (MAB258), WT (CB15N), WT^pitA (MAB257). C PhoB regulon, obtained by comparison of the two gene sets defined in (A) and (B). The red frame indicates the genes that are robustly regulated by PhoB, independently of the cytoplasmic phosphate concentration. D Clustering analysis comparing the expression levels of the 47 genes in the PhoB regulon. White color represents the average transcript level of each gene among the tested condition. Red and blue color indicates an increase or decrease, respectively, in the transcript levels compared to the average. Normalized logCPM values were used in each case, leading to a fixed range of values for all genes. S1 and S2 indicate the two replicates analyzed for each strain.