Fig. 9: Induction kinetics of phosphate-regulated genes upon phosphate deprivation.

Wild-type (CB15N) cells harboring reporter plasmids that carry fusions of the pstS (pMAB34), pstC (pMAB218), CCNA_00486 (pMAB105), ppk1 (pMAB106), CCNA_01606 (pJR16), CCNA_01636 (pMAB226), ldpA (pMAB219), stpX (pMAB222) and CCNA_00594 (pMAB223) promoter regions to the lacZ gene were grown in PYE medium and then diluted (1:20) into M2G−P medium. Samples were taken at the respective time points and subjected to β-galactosidase activity assays. Data represent the mean (± SD) of three independent measurements.