Table 1 Characteristics of the indicated strains with respect to the cytoplasmic phosphate level, the activation state of PhoB and the presence of PitA in phosphate-replete conditions

From: The cytoplasmic phosphate level has a central regulatory role in the phosphate starvation response of Caulobacter crescentus

Feature

Strain

WT

WTpitA

ΔphoB

ΔphoBpitA

ΔpstS

ΔpstSpitA

Intracellular Pia

High

High

Markedly reduced

High

Very low

High

PhoB activityb

Basal

Basal

OFF

OFF

ON

ON

PitA presence

-

+

-

+

-

+

  1. The strains shown are WT (CB15N), WTpitA (MAB257), ΔphoB (JK2), ΔphoBpitA (MAB258), ΔpstS (JK158) and ΔpstSpitA (MAB259).
  2. aBased on the level of pstSCAB expression21,22 and the results of phosphate transport assays performed in this (Fig. 3) and previous22,25 work.
  3. bBased on the results of reporter gene assays (Fig. 6) and transcriptome analyses performed in this (Supplementary Data 1) and previous21,22 work.
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