Fig. 5: Real-time PCR of G. violaceus PCC7421.

Line plot graph comparing relative quantification values for each condition. a After three days of adaptation to dark conditions, it was measured by irradiating light (red line plot graph), and samples maintaining dark conditions were compared to controls (black line plot graph). GR and GvTcR transcriptions were confirmed using PCR and the prepared primer sets. The bottom panel represents a bar graph comparing dark and light conditions at 7 h. n = 4 biologically independent samples. b After three days of adaptation to light conditions, it was measured in dark conditions (blackline plot graph). It was compared through a red line plot graph maintaining light conditions. The bottom panel represents a bar graph comparing dark and light conditions at 7 h. n = 4 biologically independent samples. All samples with multiple n’s are labeled with an error bar. The standard error is calculated by dividing the standard deviation by the square root of number of measurements.