Fig. 1: Minimum inhibitory concentration (MIC) analysis of EstX-carrying Escherichia coli and macrolide esterase function confirmation using inhibition zone analysis.
From: The global distribution of the macrolide esterase EstX from the alpha/beta hydrolase superfamily
a MIC analysis of 13 macrolides antibiotics for Escherichia coli carrying either pET28a or pET28a-EstX. MIC values were determined using the broth microdilution method. b Plate inhibition zone sizes of 13 macrolides antibiotics before and after EstX hydrolysis. Agar plates were supplemented with Staphylococcus aureus ATCC 25923 to evaluate the impact of EstX-mediated hydrolysis on antibiotic activity. Data are presented as the mean ± SD of independent experiments. n  =  3 biologically independent experiments. c Plate inhibition zone analysis of 13 macrolide antibiotics against S. aureus ATCC 25923, both before and after EstX hydrolysis.
