Fig. 2: Time-dependent plate inhibition zone analysis and electrospray ionization mass spectrometry (ESI–MS) analysis of the hydrolyzed products by EstX.
From: The global distribution of the macrolide esterase EstX from the alpha/beta hydrolase superfamily
a Plate inhibition zone size analysis of four macrolide antibiotics (tylosin, tildipirosin, tilmicosin and leucomycin A5) following hydrolysis by the EstX enzyme at various time points (0, 10, 30, 60, 90, 120 min). The diameter of inhibition zones was measured to evaluate the time-dependent degradation of the antibiotics by EstX. Data are presented as the mean ± SD of independent experiments. n = 3 biologically independent experiments. b Plate inhibition zone analysis of four macrolide antibiotics against Staphylococcus aureus ATCC 25923 following hydrolysis by EstX. c–f Electrospray ionization mass spectrometry (ESI–MS) analysis of the products before and after EstX hydrolysis of tylosin, tildipirosin, tilmicosin, and leucomycin A5. The hydrolysis sites of the macrolide antibiotics are indicated by circles, where red indicates before hydrolysis and green indicates after hydrolysis.
