Fig. 1: C. albicans N-glycan, LPS, and anesthetics induce mouse death.
From: An immune-adrenergic pathway induces lethal levels of platelet-activating factor in mice
a–f Survival rates of Balb/c mice i.v. injected (a) with or without (n = 16 and n = 15 female mice, respectively) the mixed anesthetics (medetomidine 6 μg/20 g mouse body weight, midazolam 80 μg/20 g and butorphanol 100 μg/20 g) in the presence of LPS (15 µg/20 g) and J-1012 N-glycan (200 µg/20 g) (b) with phosphate buffered saline (PBS), J-1012 N-glycan (0, 100, 200 and 400 µg/20 g) in the presence of the mixed anesthetics and LPS (n = 8, 9, 10 and 10 female mice, respectively), (c) with or without LPS in the presence of J-1012 N-glycan (400 µg/20 g) and the mixed anesthetics (n = 10 female mice). d Survival rates of Balb/c and Dectin-1KO mice i.v. injected with the mixed anesthetics, LPS and J-1012 N-glycan (400 µg/20 g, n = 9 female mice). e Survival rates of Balb/c mice i.v. injected with PBS, medetomidine (18 µg/20 g), midazolam (240 µg/20 g) or butorphanol (300 µg/20 g) in the presence of LPS and J-1012 N-glycan (200 µg/20 g, n = 9 female mice), (f) with the mixed anesthetics, LPS and J-1012 N-glycan (400 µg/20 g) in the presence of atipamezole (12 μg/20 g) (n = 20 female mice). a–f All data shown are compiled from two or three independent experiments. Data were analyzed by the (a–e) Wilcoxon test and (f) Log Rank test. N.S. not significant.
