Fig. 3: N-glycan of C. albicans upregulates production of platelet activating factor leading to the lethal damage.

a, b Survival rates of Balb/c and CARD9KO and Dectin-2KO mice were analyzed with (a) the mixed anesthetics, LPS, and J-1012 N-glycan (400 μg/20 g, n = 4 male + 4 female mice), or (b) dexmedetomidine (9 μg/20 g) and C. stellatoidea N-glycan (200 μg/20 g, n = 5 male + 5 female mice). c, d Analyses of (c) PAF and (d) lyso-PAF in spleen and liver. Ten min after of injection with dexmedetomidine, LPS and C. stellatoidea N-glycan (200 μg/20 g), spleen and liver were analyzed by LC/MS (n = 3 female mice). e Survival rates of C57BL/6 (n = 4 male +8 female mice) and LPLAT9/LPCAT2KO (n = 3 male +7 female mice) mice after i.p. injection with dexmedetomidine and i.v. injection with LPS and C. stellatoidea N-glycan (200 μg/20 g). f, g Balb/c mice were injected with WEB 2086, and survival rates of the mice were analyzed with (f) J-1012 N-glycan (400 μg/20 g) and the mixed anesthetics (n = 10 female mice) or (g) C. stellatoidea (200 μg/20 g) N-glycan and dexmedetomidine (n = 14 female mice). a, b, e, f, g All data shown are compiled from two or three independent experiments by the Wilcoxon test. N.S., not significant. c, d Experiments were repeated twice, and representative results are shown. Data were analyzed by Student’s t-test, and expressed as the mean ± standard deviation (SD). N.S. not significant.