Table 1 Sequencing statistics of holo-2bRAD libraries of gill/feces and control samples in scallop and shrimp
From: Tracking the hologenome dynamics in aquatic invertebrates by the holo-2bRAD approach
Scallop gill | Scallop feces | |||||
---|---|---|---|---|---|---|
Muscle | Rep 1 | Rep 2 | Muscle | Rep 1 | Rep 2 | |
Raw reads | 11,356,587 | 10,838,115 | 10,827,205 | 10,096,799 | 10,496,678 | 10,533,265 |
High-quality reads | 11,143,083 | 10,630,023 | 10,556,324 | 9,971,598 | 10,203,820 | 10,142,807 |
High-quality reads rate | 98.41% | 97.15% | 97.05% | 98.76% | 97.21% | 97.05% |
HQ tags with the restriction site | 9,211,275 | 9,050,087 | 8,885,492 | 9,043,375 | 8,311,095 | 8,302,067 |
Mapping rate (microorganism) (%) | 0.01% | 2.32% | 2.30% | 0.01% | 10.06% | 10.13% |
Mapping rate (scallop) (%) | 99.99% | 97.68% | 97.70% | 99.99% | 89.94% | 89.87% |
Shrimp gill | Shrimp feces | |||||
---|---|---|---|---|---|---|
Sample 1 | Sample 2 | Sample 3 | Sample 1 | Sample 2 | Sample 3 | |
Raw reads | 76,359,818 | 71,720,596 | 65,356,084 | 76,729,108 | 75,291,073 | 62,540,880 |
High-quality reads | 75,747,320 | 71,352,591 | 64,855,236 | 76,268,255 | 74,939,215 | 62,309,791 |
High-quality reads rate | 99.20% | 99.49% | 99.23% | 99.40% | 99.53% | 99.63% |
HQ tags with the restriction site | 65,382,266 | 68,276,165 | 58,286,956 | 57,580,070 | 62,874,355 | 49,927,225 |
Mapping rate (microorganism) (%) | 5.26% | 5.18% | 4.69% | 9.67% | 11.12% | 9.33% |
Mapping rate (scallop) (%) | 94.74% | 94.82% | 95.31% | 90.33% | 88.88% | 90.67% |