Fig. 1: Pwt2 is a locus conditioning aggressiveness and containing avirulence gene ACE1.
From: The ACE1 secondary metabolite gene cluster is a pathogenicity factor of wheat blast fungus
a Production of a BC1F1 population for mapping of Pwt2. b Genetic map around the Pwt2 locus constructed using the BC1F1 population. Numbers of recombinants are in parentheses. c Physical maps of the Pwt2 locus in Br48 (MoT), PO12-7301-2 (MoO), and GFSI1-7-2 (MoS). Blue arrows/arrowheads, genes; grey boxes, transposable elements; pink vertical line, a nonsense mutation (Tyr1114 to stop); arrows/arrowheads drawn with dotted lines, missing genes; ZT3-8-D/ZT-1-1-A, BAC clones of Br48; pACE1Br48, a plasmid clone containing a fragment subcloned from ZT1-1-A. Shown in the parentheses are the number of 54N1 transformants showing the pwt2 phenotype (G+)/number of transformants tested. d Complementation test using F1 culture 54N1. Primary leaves of wheat cv. N4 were inoculated with Br48, 54N1 (showing the PWT2 phenotype, G-), and 54N1 transformants carrying pACE1Br48 (54N1 + pACE1Br48) or empty vector (54N1 + EV), and incubated for 5 days at 22 °C. N = 26 (for 54N1 + pACE1Br48 #35) or 27 (for the other strains) biologically independent samples. e, f Disruption assay using wild isolate Br48. Primary leaves of wheat cv. N4 (e) and barley cv. H.E.S.4 (f) were inoculated with Br48, its ACE1-knockout mutants (Br48ΔACE1), and transformants of Br48ΔACE1(#112) carrying pACE1Br48 (Br48ΔACE1 + ACE1Br48), and incubated for 5 days at 22 °C. N = 17 (for Br48ΔACE1 #112 in (f)) or 18 (for the other strains in (e and f)) biologically independent samples. The boxplots in (d–f) show the percentage of lesion area in three independent experiments. Center lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend to 1.5x the interquartile range from the 25th and 75th percentiles. Different letters indicate significant differences determined by Dunn’s test at the 5% level.
