Fig. 3: Conservation of the ACE1 cluster in P. oryzae pathotypes correlates with its significance for their aggressiveness on host species.
From: The ACE1 secondary metabolite gene cluster is a pathogenicity factor of wheat blast fungus
a Heatmap showing the identity of protein sequences of the ACE1 cluster genes (x-axis) among P. oryzae isolates (y-axis). Their original hosts are indicated on the left with colored tiles. Shown in bold are representative isolates used in (b–e). b Pathogenicity of MoO isolate Ken53-33 (Ken) and its ACE1-knockout mutants (KenΔACE1) on rice (cv. Aichi–Asahi) and barley (cv. H.E.S.4). c Pathogenicity of MoS isolate IN77-20-1-1 (IN) and its ACE1-knockout mutants (INΔACE1) on foxtail millet (cv. Aka-awa) and barley (cv. Nigrate). Nigrate was used as a common host because IN77-20-1-1 was avirulent on H.E.S.4. d Pathogenicity of MoE isolate MZ5-1-6 (MZ) and its ACE1-knockout mutants (MZΔACE1) on finger millet (cv. Purna) and barley (cv. H.E.S.4). e Pathogenicity of MoL isolate TP2 and its ACE1-knockout mutants (TP2ΔACE1) on perennial ryegrass (cv. Friend) and barley (cv. H.E.S.4). In (b–e), inoculated seedlings were incubated at 26 °C (in rice) or 22 °C (the other plants) for 4–5 days. A compatible interaction leads to complete shriveling in barley, foxtail millet, finger millet, and perennial ryegrass, but does not in rice because fourth leaves were used in rice according to the standard protocol of rice infection assay with MoO.
