Fig. 4: ACE1 alleles of MoT isolates function as avirulence genes corresponding to rice resistance gene Pi33.
From: The ACE1 secondary metabolite gene cluster is a pathogenicity factor of wheat blast fungus
a A maximum likelihood tree of ACE1 alleles inferred from protein sequences. ACE1 alleles are represented by names of isolates in which they were detected. Pathotypes of the isolates are color-coded as in Fig. 3a, i.e., MoT (blue), MoL (purple), MoE (red), MoS (light green), and MoO (green). Final designations of representative ACE1 alleles are shown in parentheses. An ACE1 homolog in P. grisea isolate Dig41 was used as an outgroup. b Effect of the disruption of ACE1 alleles on aggressiveness of MoT isolates, Br118.2 and T109. Primary leaves of wheat cv. N4 were inoculated with Br118.2, T109, and their ACE1-knockout mutants (Br118.2ΔACE1 and T109ΔACE1), and incubated for 5 days at 22 °C. c Reactions of rice and barley to transformants of MoO isolate PO12-7301-2 (PO) carrying various ACE1 alleles. Fourth leaves of rice cv. Aichi–Asahi (pi33) and cv. Bala (Pi33) and primary leaves of barley cv. Nigrate were inoculated with PO, its transformants carrying an empty vector (PO + EV), ACE1 of MoO isolate Ken53-33 (PO + ACE1Ken), and the ACE1 alleles of MoT isolates Br48, Br118.2, and T109 (PO + ACE1Br48, PO + ACE1Br118.2, and PO + ACE1T109), and incubated at 26 °C (rice) or 22 °C (barley) for 5 days. Similar results were obtained in three independent experiments.
