Fig. 1: The B-Raf preference of the H-Ras nanocluster scaffold Gal1 emerges within the RBD.
From: Identification of an H-Ras nanocluster disrupting peptide
a Schematic of our model for Gal1 stabilized H-Ras nanocluster. b Dose-dependent effect of human Gal1 expression (48 h) on H-RasG12V nanoclustering-BRET (donor:acceptor plasmid ratio = 1:5); n = 4. c BRET-titration curves of the Gal1/ Gal1-interaction as compared to that of dimer-interface mutated N-Gal1. RLuc8-Gal1 was titrated with GFP2 as a control (black); n = 3. d BRET-titration curves of the Gal1-interaction with the RBDs of A-, B-, and C-Raf; n = 3. e Split-luciferase KinCon B-Raf biosensor response after expression of SNAP-H-RasG12V or Gal1; n = 3.
