Fig. 3: The L5UR and L5UR-SNAP peptides disrupt H-RasG12V nanoclustering.
From: Identification of an H-Ras nanocluster disrupting peptide
a Schematics of L5UR derived constructs expressed in cellular assays. The stretch of the core peptide is highlighted in blue, loss-of-function mutations are indicated red. b Effect of expression of L5UR constructs (48 h) on Gal1/B-RBD BRET (donor:acceptor plasmid ratio = 1:10); n = 3. c Effect of L5UR construct expression (48 h) on H-RasG12V nanoclustering-BRET with co-transfection of 200 ng Gal1 plasmid (donor:acceptor plasmid ratio = 1:5); n = 3. Statistical comparison was done against the SNAP-only sample. d Electron microscopy-based analysis of H-RasG12V nanoclustering in BHK cells showing the effects of L5UR-construct expression and controls; n = 15. Higher Lmax values indicate higher nanoclustering. e Immunoblot data from pull-down assays with L5UR-SNAP and control constructs from HEK cells co-expressing Gal1 with example blots (left) and quantification of repeat data (right); n = 4 (left).
