Fig. 4: The TAT-tagged L5URcore peptide disrupts H-RasG12V nanoclustering.
From: Identification of an H-Ras nanocluster disrupting peptide
a Schematics of TAT-functionalized L5URcore-derived peptides and controls as applied in cellular assays. Loss-of-function mutations of L5UR are indicated in red. Non-TAT peptides are acetylated at the N-terminus. b, c Effect of cell-penetrating derivatives of L5URcore and control peptides on Gal1/B-RBD BRET (b donor:acceptor plasmid ratio = 1:10; n ≥ 2) or H-RasG12V nanoclustering-BRET (c donor:acceptor plasmid ratio = 1:5, co-transfection of 200 ng Gal1 plasmid; n = 3). After 24 h expression of plasmids, peptides were added to cells at specified concentrations and incubated for 2 h.
