Fig. 2: Overview of differentially expressed metabolites identified through pairwise comparisons at key timepoints during sorghum seed development. | Communications Biology

Fig. 2: Overview of differentially expressed metabolites identified through pairwise comparisons at key timepoints during sorghum seed development.

From: Transcriptome and metabolome analyses reveal regulatory networks associated with nutrition synthesis in sorghum seeds

Fig. 2

a Morphological transitions in BTx623 sorghum seeds at five critical timepoints (5, 10, 15, 20, and 25 dpa) subjected to metabolome analysis. b Volcano plot displaying metabolite features, with numbers indicating significantly upregulated [log2 (fold-changes) ≥ 1.5; q-values ≤ 0.05] and downregulated [log2 (fold-changes) ≤ -1.5; q-values ≤ 0.05] metabolites in the specified comparisons. Gray-dashed lines represent the q-value and fold-change filter. c–f Categorization of upregulated and downregulated metabolic pathways according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) for different comparison groups (10 Vs. 5 dpa, 15 Vs. 5 dpa, 20 Vs. 5 dpa, and 25 Vs. 5 dpa). Node colors indicate p-values, with white and red denoting lower and higher p-values, respectively. Node radii correspond to pathway impact values, with smaller and larger radii indicating lower and higher impact values, respectively.

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